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mt DNA was recognized by cyclic-GMP-AMP synthase (c GAS) in the DC cytosol, contributing to type I interferon (IFN) production and antitumor adaptive immunity.

Thus, our findings have demonstrated how tumor cells inhibit innate sensing in DCs and suggested that the CD47-SIRPα axis is critical for DC-driven antitumor immunity.

Cells were obtained using Institutional Review Board (IRB)-approved consent forms and protocols.

Standard PBMCs (Catalog #70025) include the following characterization criteria: cell count, viability, donor virus testing, age, sex, ethnicity, weight, height and smoking status.

Characterized PBMCs (Catalog #70047) include the same information as Standard PBMCs in addition to the following characterization criteria: high-resolution HLA typing (A, B, C, DRB1, DRB3/4/5 and DQB1), ADCC status, ELISpot reactivity and cell proliferation index.

HLA-A2 PBMCs (Catalog #70048) are selected for HLA-A2 positive donors and include the same characterization criteria as Standard PBMCs and Characterized PBMCs.

Proof of drug-target engagement in physiologically-relevant contexts is a key pillar of successful therapeutic target validation.

PB was collected using acid-citrate-dextrose solution A (ACDA), acid-citrate-dextrose solution B (ACDB), citrate-phosphate-dextrose (CPD), or sodium heparin (Na-Hep) as the anticoagulant.Here we demonstrate that NEUROG3 is phosphorylated within the nucleus on serine 183, which catalyzes its hyperphosphorylation and proteosomal degradation.During progression through the progenitor cell cycle, NEUROG3 phosphorylation is driven by the actions of cyclin-dependent kinases 2 and 4/6 at G1/S cell-cycle checkpoint.ATPS droplet formation is achieved by applying weak hydrostatic pressures, with liquid-filled pipette tips as fluid columns at the inlets, to introduce low speed flows to the flow focusing junction.To control the size of the droplets, we systematically vary the interfacial tension and viscosity of the ATPS fluids and adjust the fluid column height at the fluid inlets.

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